杜丹
教授、博士生导师、海外高层次青年人才、“闽江学者”特聘教授、我校“南强青年拔尖A类人才” 学科（方向）：肿瘤细胞生物学 邮 箱：dandu@xmu.edu.cn |  |
研究领域：
主要研究方向（不限于）：
1. 肿瘤免疫：运用基于CRISPRn/i（敲出、抑制）高通量体内外筛选平台，运用小鼠体内成瘤模型研究：(1) 肿瘤细胞向淋巴结转移的分子机制; (2) 肿瘤细胞逃逸免疫系统的分子机制; (3) 免疫细胞在肿瘤微环境浸润、激活（失活）的分子机制。
2. 肠道微生态在消化道疾病中的功能：运用基于CRISPRn/i（敲出、抑制）高通量体内外筛选平台，(1) 鉴定致病菌作用于宿主细胞的靶点；(2) 解析宿主细胞应答致病菌的分子机制；(3) 探索肠道微生态、肠道免疫、肠道神经和肠细胞的相互作用机制。
3. 肿瘤电场治疗研究：在分子、细胞、组织和动物水平，研究不同种类肿瘤对交流可变电场（TTfields）治疗和联合治疗条件下的应答效率及机制。
4. 基于CRISPR的检测技术和平台：开发基于CRISPR的快速、高效、灵敏和精确的多功能现场诊断方法。
学习经历：
1998~2002 南京大学生物化学系 理学学士；
2002~2009 中国科学院上海生化细胞所 理学博士；
2005.3~2005.12 德国马克思普朗克生物化学研究所 访问博士；
2006.1~2006.4 丹麦哥本哈根分子病理研究所 访问博士；
工作经历：
2009~2010 中国科学院上海生化细胞所 研究助理；
2010~2015 美国加州大学旧金山分校细胞和组织学系 博士后；
2015~2018 美国加州大学旧金山分校细胞和组织学系 associate specialist；
2014.10~2015.3美国斯坦福大学生物工程学系 访问学者；
2015.5~2017.4 美国格拉德斯通研究所 访问学者；
社会及学术兼职：
细胞生物学学会细胞结构与功能分会 委员
福建省生物工程学会 理事
代表性成果：
1. Xiao Wang#, Qiao Rao#, Zhengrong Lu#, Xiaobao Deng, Rong Shen, Rui Wang, Weiyi Dong, Xianhua Qi, Zhen Jin, Youzhi Tang*, Dan Du*. Rapid and sensitive Cas13a/Cas12a-based one-pot dual-target strategy to detect monkeypox virus and its co-infected viruses. Science Bulletin., 2023, in press (*: co-corresponding author);
2. Dan Du*, Yoko Katsuno, Dominique Meyer, Erine H. Budi, Si-Han Chen, Hongjun Wang, Hartmut Koeppen, Rosemary Akhurst, Rik Derynck*. Smad3‐mediated recruitment of the methyltransferase SETDB1/ESET controls Snail1 expression and epithelial–mesenchymal transition. EMBO Reports., 2017 Dec 12th; 19(1), 135-155 (*: co-corresponding author);
3. Dan Du#, Assen, Roguev#, David E Gordon，Meng Chen, Si-Han Chen, John Paul Shen, Prashant Mali, Trey Ideker, Stanley Qi, Nevan Krogan. Genetic interaction mapping in mammalian cells using CRISPR interference. Nat. Methods., 2017 May 8th; 14(6), 577–580 (#: co-first author)；
4. Dan Du, Feilai Xu, Lihou Yu, Chenyi Zhang, Xuefeng Lu, Haixin Yuan, Qin Huang, Fan Zhang, Xunwei Wu, Zhe Zhang, Xueliang Zhu, Xiaohui Zhang, Zhengjun Chen. Tight junction protein occludin regulates directional migration of epithelial cells. Dev Cell., 2010 Jan 19th, 18 (1), 52-63；
5. Dan Du, Stanley Qi. CRISPR Technology for Genome Activation and Repression in Mammalian Cells. Cold Spring Harb Protoc. 2016 Jan 4th; (1), pdb. prot090175；
6. Dan Du, Stanley Qi. An Introduction to CRISPR Technology for Genome Activation and Repression in Mammalian Cells. Cold Spring Harb Protoc. 2016 Jan 4th ; (1), pdb. top086835；
7. Dan Du, Esben Pedersen, Zhipeng Wang, Richard Karlsson, Zhengjun Chen, Xunwei Wu, Cord Brakebusch. (2009) Cdc42 is crucial for the maturation of primordial cell junctions in keratinocytes independent of Rac1. Exp Cell Res., 315, 1480-1489.
主持过的重要课题：
1. 科技部重大研发计划（微生物机制研究及微生物宿主互作靶标的筛选，2022YFA1304002，2022-2027，440万元）
2. 国家自然科学基金面上项目（KMT1E-s在乳腺癌治疗耐受和复发中的功能及机制研究，81972823，2020-2023，60万元）
3. 国家自然科学基金面上项目（RNA结合蛋白Strap调控浆细胞分化和肿瘤生长的机制研究，82372788，2024-2027，73万元）
4. 闽江学者启动经费（200万元）
5. Human Frontier Science Program (HFSP) fellowship (as PI), “Role of the methyltransferase ESET in TGF-beta signaling and EMT”, 2011-2014. This award is around $160,980.
6. The Leukemia & Lymphoma Society FELLOW Award (as PI), “Functional crosstalk between SMYD3 and TGF-beta signaling to control cellular senescence”, 2011-2014. This award is around $165,000.
所有论文列表：
1. Xiao Wang#, Qiao Rao#, Zhengrong Lu#, Xiaobao Deng, Rong Shen, Rui Wang, Weiyi Dong, Xianhua Qi, Zhen Jin, Youzhi Tang*, Dan Du*. Rapid and sensitive Cas13a/Cas12a-based one-pot dual-target strategy to detect monkeypox virus and its co-infected viruses. Science Bulletin., 2023, in press (*: co-corresponding author);
2. Tao Luo, Lican Zheng, Dong-Yang Chen, Chen Zhang, Sirui Liu, Chongjie Jiang, Yu Xie, Dan Du, Wei Zhou. Implantable microfluidics: methods and applications. Analyst. 2023 Sep 25th;148(19):4637-4654. doi: 10.1039/d3an00981e.
3. Xiao Wang, Rui Wang, Zhao-Sheng Zhang 3, Guang-Yu Zhang, Zhen Jin, Rong Shen, Dan Du*, You-Zhi Tang*. Semisynthetic pleuromutilin antimicrobials with therapeutic potential against methicillin-resistant Staphylococcus aureus by targeting 50S ribosomal subunit. Eur J Med Chem. 2022 Jul 5th;237:114341. doi: 10.1016/j.ejmech. 2022.114341 (*: co-corresponding author).
4. Si-Yu Huang, Xiao Wang, Ding-Yi Shen, Fang Chen , Guang-Yu Zhang, Zhe Zhang, Kang Li, Zhen Jin, Dan Du, You-Zhi Tang. Design, synthesis and biological evaluation of novel pleuromutilin derivatives as potent anti-MRSA agents targeting the 50S ribosome. Bioorg Med Chem. 2021 May 15th;38:116138. doi: 10.1016/j. bmc. 2021.116138.
5. Hongfeng Zhang, Yujuan Hong, Weijie Yang, Ruimin Wang, Ting Yao, Jian Wang, Ke Liu, Huilong Yuan, Chaoqun Xu, Yuanyuan Zhou, Guanxian Li, Lishan Zhang, Hong Luo, Xian Zhang, Dan Du, Hao Sun, Qiuyang Zheng, Yun-Wu Zhang, Yingjun Zhao, Ying Zhou, Huaxi Xu, Xin Wang SNX14 deficiency-induced defective axonal mitochondrial transport in Purkinje cells underlies cerebellar ataxia and can be reversed by valproate. Natl Sci Rev. 2021 Feb 10th;8(7):nwab024. doi: 10.1093/nsr/nwab024.
6. Xiao Wang, Ling-Ling Xu, Xiang-Yi Zuo, Jia-Wen Lin, Zhen Jin 1, Rong Shen, Dan Du*, You-Zhi Tang*. Rapid detection of the New Delhi metallo-β-lactamase (NDM) gene by recombinase polymerase amplification. Infect Genet Evol. 2021 Jan;87:104678. doi: 10.1016/j.meegid.2020.104678(*: co-corresponding author).
7. Wei Wang, Ru-Feng Yue, Zhen Jin, Li-Min He, Rong Shen, Dan Du*, You-Zhi Tang*. Efficiency comparison of apigenin-7-O-glucoside and trolox in antioxidative stress and anti-inflammatory properties. J Pharm Pharmacol. 2020 Nov;72(11):1645-1656. doi: 10.1111/jphp.13347(*: co-corresponding author).
8. Li Zhong, Ying Xu, Ren-Gong Zhuo, Tingting Wang, Kai Wang, Ruizhi Huang, Daxin Wang, Yue Gao, Yifei Zhu, Xuan Sheng, Kai Chen, Na Wang, Lin Zhu, Dan Can, Yuka Martens, Mitsuru Shinohara, Chia-Chen Liu, Dan Du, Hao Sun, Lei Wen, Huaxi Xu, Guojun Bu, and Xiaofen chen. Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in a mouse model of Alzheimer’s disease. Nature Communications., 2019 Mar 25th;10(1):1365. doi: 10.1038/s41467-019-09118-9.
9. Si‐Han Chen, Gwendolyn M Jang, Ruth Hüttenhain, David E Gordon, Dan Du, Billy W Newton, Jeffrey R Johnson, Joseph Hiatt, Judd F Hultquist, Tasha L Johnson, Yi‐Liang Liu, Lily A Burton, Jordan Ye, Kurt M Reichermeier, Robert M Stroud, Alexander Marson, Jayanta Debnath, John D Gross, Nevan J Krogan#. CRL4AMBRA1 targets Elongin C for ubiquitination and degradation to modulate CRL5 signaling. EMBO J., 2018 Aug 30th , doi: 10.15252/embj.201797508
10. Dan Du*, Yoko Katsuno, Dominique Meyer, Erine H. Budi, Si-Han Chen, Hongjun Wang, Hartmut Koeppen, Rosemary Akhurst, Rik Derynck*. Smad3‐mediated recruitment of the methyltransferase SETDB1/ESET controls Snail1 expression and epithelial–mesenchymal transition. EMBO Reports., 2017 Dec 12th; 19(1), 135-155 (*: co-corresponding author). doi: 10.15252/embr.201744250
11. Dan Du#, Assen, Roguev#, David E Gordon，Meng Chen, Si-Han Chen, John Paul Shen, Prashant Mali, Trey Ideker, Stanley Qi, Nevan Krogan. Genetic interaction mapping in mammalian cells using CRISPR interference. Nat. Methods., 2017 May 8th; 14(6), 577–580 (#: co-first author). doi:10.1038/nmeth.4286
12. John Paul Shen, Dongxin Zhao, Roman Sasik, Jens Luebeck, Amanda Birmingham, Ana Bojorquez-Gomez, Katherine Licon, Kristin Klepper, Daniel Pekin, Alex N Beckett, Kyle Salinas Sanchez, Alex Thomas, Chih-Chung Kuo, Dan Du, Assen Roguev, Nathan E Lewis, Aaron N Chang, Jason F Kreisberg, Nevan Krogan, Lei Qi, Trey Ideker, Prashant Mali. Combinatorial CRISPR–Cas9 screens for de novo mapping of genetic interactions. Nat. Methods., 2017 May 8th; 14(6), 573–576. doi:10.1038/nmeth.4225.
13. Dan Du, Stanley Qi. CRISPR Technology for Genome Activation and Repression in Mammalian Cells. Cold Spring Harb Protoc. 2016 Jan 4th; (1), pdb. prot090175. doi:10.1101/pdb.prot090 175.
14. Dan Du, Stanley Qi. An Introduction to CRISPR Technology for Genome Activation and Repression in Mammalian Cells. Cold Spring Harb Protoc. 2016 Jan 4th ; (1), pdb. top086835. doi:10.1101/pdb.top086835.
15. Pinglong Xu, Samantha Bailey-Bucktrout, Ying Xi, Daqi Xu, Dan Du, Qian Zhang, Weiwen Xiang, Jianming Liu, Andrew Melton, Dean Sheppard, Harold A. Chapman, Jeffrey A. Bluestone, Rik Derynck. Innate Antiviral Host Defense Attenuates TGF-β Function through IRF3-Mediated Suppression of Smad Signaling. Mol Cell., 2014 Dec 18th; 56(6), 723–737. doi: https://doi.org/ 10.1016/j.molcel.2014.11.027.
16. Dan Du, Feilai Xu, Lihou Yu, Chenyi Zhang, Xuefeng Lu, Haixin Yuan, Qin Huang, Fan Zhang, Xunwei Wu, Zhe Zhang, Xueliang Zhu, Xiaohui Zhang, Zhengjun Chen. Tight junction protein occludin regulates directional migration of epithelial cells. Dev Cell., 2010 Jan 19th, 18 (1), 52-63. doi: https://doi.org/10.1016/j.devcel.2009.12.008.
17. Dan Du, Esben Pedersen, Zhipeng Wang, Richard Karlsson, Zhengjun Chen, Xunwei Wu, Cord Brakebusch. (2009) Cdc42 is crucial for the maturation of primordial cell junctions in keratinocytes independent of Rac1. Exp Cell Res., 315, 1480-1489. (doi:10.1016/j. yexcr.2008.11.012.)
18. Xuefeng Lu*, Xiujing Feng*, Xiaobo Man, Guang Yang, Liang Tang, Dan Du, Fan Zhang, Haixin Yuan, Qin Huang, Zhe Zhang, Yinkun Liu, Dennis Strand, Zhengjun Chen. (2009) Aberrant Splicing of Hugl-1, the Human Homologue of Drosophila Tumor Suppressor Gene lgl, is Associated with Hepatocellular Carcinoma Progression. Clin Cancer Res., 15(10), 3287-3296 (*: co-first author).
19. Haixin Yuan*, Hong Zhang*, Xunwei Wu, Zhe Zhang, Dan Du, Wenchao Zhou, Shuhua Zhou, Cord Brakebusch, Zhengjun Chen. (2009) Hepatocyte-Specific Deletion of Cdc42 Results in Delayed Liver Regeneration after Partial Hepatectomy in Mice. Hepatology., 49,240-249. (*: co-first author).
20. Zhou Ying*, Fang Longhou*, Du Dan, Zhou Wenchao, Feng Xiujing, Chen Jiwu, Chen Zhengjun. (2008) Proteome identification of binding-partners interacting with cell polarity protein Par3 in Jurkat cells. Acta Biochim Biophys Sin (Shanghai)., 8:729-39. (*: co-first author).
21. Longhou Fang, YiGuo Wang, Dan Du, Guang Yang, Tim Tak Kwok, Siu Kai Kong, Benjamin Chen, David J. Chen, and Zhengjun Chen. (2007) Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair. Cell Res., 17: 100-116.
22. YiGuo Wang, Dan Du, Longhou Fang, Guang Yang, Chenyi Zhang, F Lottspeich, Axel Ullrich, Zhengjun Chen. (2006) Tyrosine Phosphorylated Par3 Regulates Epithelial Tight Junction Assembly Promoted by EGFR Signaling. EMBO J., 5:5058-5070.
23. YiGuo Wang*, Rongxia Li*, Dan Du, Chenyi Zhang, Haixin Yuan, Rong Zeng, and Zhengjun Chen. (2006) Proteomic Analysis Reveals Novel Molecules Involved in Insulin Signaling Pathway. J Proteome. Res., 5:846-855 (*: co-first author).
